P53 Status Of Breast Cancer Cell Lines

Germ line mutations in p53 occur in a high proportion of individuals with the li fraumeni cancer susceptibility syndrome which confers an increased risk of breast cancer this implies an important role for p53 inactivation in mammary carcinogenesis and the structure and expression of p53 has been widely studied in breast cancer.

P53 status of breast cancer cell lines.

Strikingly we found discrepancies in the p53 status for 23 88 384 of cell lines for which the p53 status was established independently in two laboratories. Wild type p53 was found in six cell lines and various p53 aberrations in the remaining twenty. Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. Cell lines with p53 mutations missense or frameshift more information is available in the handbook.

Codon position 1 to 393. Nsclc cancer cell lines. Breast cancer cell lines. Most cell lines were established in late 1970s.

Gastric cancer cell lines. P53 activity is modulated by numerous posttranslational modifications association with protein. Cell lines with a controversial p53 status. Esophageal cancer cell lines.

If the mutation is a deletion or an insertion it is indicated by del or ins followed by the number of deleted or inserted bases. Knowledgebase and statistical tools for the analysis of tp53 gene mutations in human cancers. Ever since the establishment of the first breast cancer cell line bt 20 in 1958 relatively few cell lines have been obtained due to technical difficulties in extracting viable tumor cells from the surrounding stroma 12 13 and the bottleneck of long term propagation during cultivation 12 14. Mut sequence of the mutated codon.

This is a list of major breast cancer cell lines that are primarily used in breast cancer. Colorectal cancer cell lines. Brain cancer cell lines. Fasay detected temperature sensitive p53 mutations in breast cancer cell line bt474 and leiomyosarcoma cell line sk lms 1 and two independent p53 point mutations in sk ut 1 and sk lms 1 leiomyosarcoma cell lines.

Leukemia lymphoma cell lines. Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. I have to perform experiments with p53 wild type and mutant breast cancer cell lines for which i need to know their p53 status. Head and neck cancer cell lines.

The nci 60 panel. Tp53 codes for a transcription factor p53 that initiates transcription of genes involved in cell cycle arrest cellular senescence apoptosis metabolism dna repair and other processes following cellular stress. Normal base sequence of the codon in which the mutation occurred. P53 mutation in breast cancer.

Bladder cancer cell lines.